Test Code VHPROFIL Vascular Hypercoagulation Profile
Specimen Draw Volume
2.7 mL Blue - Anti Thrombin III Activity
3.0 mL Gold SST - Beta 2 Glycoprotein and Cardiolipin Antibodies
4.0 mL Lavender - Factor V Leiden
1.0 mL GRN PST - Homocysteine
5.4 mL Blue - Lupus Anticoagulant
2.7 mL Blue - Protein C Activity
2.7 mL Blue - Protein S Activity
4.0 mL Lavender - PT 20210 Gene Mutation
3.0 mL Gold SST - Antinuclear Antibody Screen, rflx Confirmation
4.0 mL Lavender - MTHFR Mutations, Blood
Processing Instructions
Anti Thrombin III Activity - Draw 2.7 ml in a Blue Top tube. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube, aliquot 1.0 mL plasma, centrifuge plasma again, and place 1 ml of platelet-poor plasma in an aliquot tube and freeze.
Beta 2 Glycoprotein and Cardiolipin Antibodies - Draw in a Gold SST tube. Allow tube to clot for 30 minutes and then centrifuge. Aliquot a minimum of 1.0 mL serum.
Factor V Leiden - Draw 4 mL in an EDTA Lavender Top tube. Minimum volume is 0.5 mLs Send separate lavender top tube for this test unopened to laboratory refrigerated.
Homocysteine - Draw in Green PST on ice, centrifuge, aliquot, and refrigerate aliquot. Gold SST are acceptable but takes longer to clot. Specimen must be kept on ice water until centrifuged. Specimen must be aliquoted within 6 hours. Failure to place sample on ice prior to separation from cells can result in a 10-20% elevation of homocysteine levels in the sample.
Lupus Anticoagulant - Draw 2.7 ml in 2 Blue Top tubes. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube and remove plasma, centrifuge plasma again, and place 1 mL of platelet-poor plasma in 2 aliquot tubes and freeze.
Protein C Activity - Draw 2.7 ml in a Blue Top tube. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube and remove plasma, centrifuge plasma again, and place 1 mL of platelet-poor plasma in an aliquot tube and freeze.
Protein S Activity - Draw 2.7 ml in a Blue Top tube. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube and remove plasma, centrifuge plasma again, and place 1 mL of platelet-poor plasma in an aliquot tube and freeze.
PT 20210 Gene Mutation - Draw 4 mL in an EDTA Lavender Top tube. Minimum volume is 0.5 mLs Send separate lavender top tube for this test unopened to laboratory refrigerated.
Antinuclear Antibody Screen, rflx Confirmation - Draw in a Gold SST tube. Allow tube to clot for 30 minutes, centrifuge, and refrigerate.
MTHFR Mutations, Blood - Draw in a EDTA Lavender Top tube. Do not centrifuge specimen. Do not transfer blood to other containers. Send Room Temp.
Stability
Anti Thrombin III Activity - Stability: Room temp. 8 hrs., Frozen 1 month.
Beta 2 Glycoprotein and Cardiolipin Antibodies - Stability: Refrigerated 7 days, >7 days freeze.
Factor V Leiden - Stability: Refrigerated
Homocysteine - Stability: Refrigerated 14 days, frozen 1 year.
Lupus Anticoagulant - Stability: Room temp 2 hrs., frozen 2 weeks.
Protein C Activity - Stability: Room temp 8 hrs., frozen (-20 C) 1 month.
Protein S Activity - Stability: Room temp 4 hrs., frozen (-20 C) 1 month.
PT 20210 Gene Mutation - Stability: Refrigerated
Antinuclear Antibody Screen, rflx Confirmation - Stability: Refrigerated 7 days, Frozen >7 days.
MTHFR Mutations, Blood - Stability: Room Temp 7 days, Refrigerated 7 days, Frozen 14 days.
CPT(s)
81241 86146 x 3 86147 x 3 85300 85613 85730 85303 85305 81240 86038 81291
Performing Location
Test performed at MercyOne Des Moines Laboratory.
Cerner Order Code
VHPROFIL
Acceptable Collection Tube(s)
Blue - Anti Thrombin III Activity
Gold SST or Red Top - Beta 2 Glycoprotein and Cardiolipin Antibodies
Lavender - Factor V Leiden
GRN PST or Gold SST- Homocysteine
Blue - Lupus Anticoagulant
Blue - Protein C Activity
Blue - Protein S Activity
Lavender - PT 20210 Gene Mutation
Gold SST or Red Top - Antinuclear Antibody Screen, rflx Confirmation
Lavender - MTHFR Mutations, Blood
Panel Includes
Anti Thrombin III Activity
Beta 2 Glycoprotein and Cardiolipin Antibodies
Factor V Leiden
Homocysteine
Lupus Anticoagulant
Protein C Activity
Protein S Activity
PT 20210 Gene Mutation
Antinuclear Antibody Screen, rflx Confirmation
MTHFR Mutations, Blood