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Test Code VHPROFIL Vascular Hypercoagulation Profile

Specimen Draw Volume

2.7 mL Blue - Anti Thrombin III Activity

3.0 mL Gold SST - Beta 2 Glycoprotein and Cardiolipin Antibodies

4.0 mL Lavender - Factor V Leiden

1.0 mL GRN PST - Homocysteine

5.4 mL Blue  - Lupus Anticoagulant

2.7 mL Blue - Protein C Activity

2.7 mL Blue - Protein S Activity

4.0 mL Lavender - PT 20210 Gene Mutation

3.0 mL Gold SST - Antinuclear Antibody Screen, rflx Confirmation

4.0 mL Lavender - MTHFR Mutations, Blood

 

Processing Instructions

Anti Thrombin III Activity - Draw 2.7 ml in a Blue Top tube. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube, aliquot 1.0 mL plasma, centrifuge plasma again, and place 1 ml of platelet-poor plasma in an aliquot tube and freeze.

Beta 2 Glycoprotein and Cardiolipin Antibodies - Draw in a Gold SST  tube. Allow tube to clot for 30 minutes and then centrifuge. Aliquot a minimum of 1.0 mL serum. 

Factor V Leiden - Draw 4 mL in an EDTA Lavender Top tube. Minimum volume is 0.5 mLs  Send separate lavender top tube for this test unopened to laboratory refrigerated.

Homocysteine - Draw in  Green PST on ice, centrifuge, aliquot, and refrigerate aliquot. Gold SST  are acceptable but takes longer to clot. Specimen must be kept on ice water until centrifuged. Specimen must be aliquoted within 6 hours. Failure to place sample on ice prior to separation from cells can result in a 10-20% elevation of homocysteine levels in the sample.

Lupus Anticoagulant - Draw 2.7 ml in 2 Blue Top tubes. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube and remove plasma, centrifuge plasma again, and place 1 mL of platelet-poor plasma in 2 aliquot tubes and freeze.

Protein C Activity - Draw 2.7 ml in a Blue Top tube. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube and remove plasma, centrifuge plasma again, and place 1 mL of platelet-poor plasma in an aliquot tube and freeze.

Protein S Activity - Draw 2.7 ml in a Blue Top tube. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube and remove plasma, centrifuge plasma again, and place 1 mL of platelet-poor plasma in an aliquot tube and freeze.

PT 20210 Gene Mutation - Draw 4 mL in an EDTA Lavender Top tube. Minimum volume is 0.5 mLs  Send separate lavender top tube for this test unopened to laboratory refrigerated.

Antinuclear Antibody Screen, rflx Confirmation - Draw in a Gold SST  tube. Allow tube to clot for 30 minutes, centrifuge, and refrigerate. 

MTHFR Mutations, Blood - Draw in a EDTA Lavender Top tube. Do not centrifuge specimen. Do not transfer blood to other containers. Send Room Temp.

 

Stability

Anti Thrombin III Activity - Stability:  Room temp. 8 hrs., Frozen 1 month. 

Beta 2 Glycoprotein and Cardiolipin Antibodies - Stability:  Refrigerated 7 days, >7 days freeze.

Factor V Leiden - Stability:  Refrigerated

Homocysteine - Stability:  Refrigerated 14 days, frozen 1 year.

Lupus Anticoagulant - Stability:  Room temp 2 hrs., frozen 2 weeks.

Protein C Activity - Stability:  Room temp 8 hrs., frozen (-20 C) 1 month.

Protein S Activity - Stability:  Room temp 4 hrs., frozen (-20 C) 1 month.

PT 20210 Gene Mutation - Stability:  Refrigerated

Antinuclear Antibody Screen, rflx Confirmation - Stability: Refrigerated 7 days,  Frozen >7 days.

MTHFR Mutations, Blood - Stability: Room Temp 7 days,  Refrigerated 7 days,  Frozen 14 days.

 

CPT(s)

81241  86146 x 3  86147 x 3  85300  85613 85730 85303 85305 81240 86038 81291

Performing Location

Test performed at MercyOne Des Moines Laboratory.

Cerner Order Code

VHPROFIL

Acceptable Collection Tube(s)

Blue - Anti Thrombin III Activity

Gold SST or Red Top - Beta 2 Glycoprotein and Cardiolipin Antibodies

Lavender - Factor V Leiden

GRN PST or Gold SST- Homocysteine

Blue - Lupus Anticoagulant

Blue - Protein C Activity

Blue - Protein S Activity

Lavender - PT 20210 Gene Mutation

Gold SST or Red Top - Antinuclear Antibody Screen, rflx Confirmation

Lavender - MTHFR Mutations, Blood

 

Panel Includes

Anti Thrombin III Activity

Beta 2 Glycoprotein and Cardiolipin Antibodies

Factor V Leiden

Homocysteine

Lupus Anticoagulant

Protein C Activity

Protein S Activity

PT 20210 Gene Mutation

Antinuclear Antibody Screen, rflx Confirmation

MTHFR Mutations, Blood