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Test Code OB HCP OB Hypercoagulable Profile

Important Note

Anticoagulant therapy effects, such as Warfarin (Coumadin), heparin, direct thrombin inhibitors (Dabigatran [Pradaxa], Argatroban [Ancova], Bivalirudin [Angiomax]), direct factor Xa inhibitors (Rivaroxaban [Xarelto], Apixaban [Eliquis], Edoxaban [Savaysa]), may result in false positive assay performance for Lupus Anticoagulant Studies, PT, PTT, TT, Anti-Xa, ATIII, Protein S / Protein C Activity. Clinical correlation with repeat testing once anticoagulant therapy has been discontinued for greater than one week is recommended if interference is suspected. 

Specimen Draw Volume

5 - Full Blues

2 - Full Lavenders

1.0 mL GRN Pst

3.0 mL Gold SST

Processing Instructions

Beta 2 Glycoprotein and Cardiolipin Antibodies - Draw in a Gold SST  tube. Allow tube to clot for 30 minutes and then centrifuge. Aliquot a minimum of 1.0 mL serum. 

Homocysteine - Draw in  Green PST on ice, centrifuge, aliquot, and refrigerate aliquot. Gold SST  are acceptable but takes longer to clot. Specimen must be kept on ice water until centrifuged. Specimen must be aliquoted within 6 hours. Failure to place sample on ice prior to separation from cells can result in a 10-20% elevation of homocysteine levels in the sample.

Lupus Anticoagulant - Draw 2.7 ml in 2 Blue Top tubes. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube and remove plasma, centrifuge plasma again, and place 1 mL of platelet-poor plasma in 2 aliquot tubes and freeze.

Factor V Leiden - Draw 4 mL in an EDTA Lavender Top tube. Minimum volume is 0.5 mLs  Send separate lavender top tube for this test unopened to laboratory refrigerated.

Anti Thrombin III Activity - Draw 2.7 ml in a Blue Top tube. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube, aliquot 1.0 mL plasma, centrifuge plasma again, and place 1 ml of platelet-poor plasma in an aliquot tube and freeze.

PT 20210 Gene Mutation - Draw 4 mL in an EDTA Lavender Top tube. Minimum volume is 0.5 mLs  Send separate lavender top tube for this test unopened to laboratory refrigerated.

Protein C Activity - Draw 2.7 ml in a Blue Top tube. Tube must be filled appropriately to the fill line to ensure accurate results. Centrifuge tube and remove plasma, centrifuge plasma again, and place 1 mL of platelet-poor plasma in an aliquot tube and freeze.

Protein S Free Antigen with Reflex - Draw in a Blue Top tube. Centrifuge tube and remove plasma, centrifuge plasma again, and place 2 mL in 2 plastic vials each containing 1.0 mL and freeze. (Double centrifuged specimens are necessary for accurate results).

 

Stability

Beta 2 Glycoprotein and Cardiolipin Antibodies  Stability: Refrigerated 7 days, >7 days freeze.

Homocysteine  Stability:  Refrigerated 14 days, frozen 1 year.

Lupus Anticoagulant  Stability:  Room temp 2 hrs., frozen 2 weeks.

Factor V Leiden   Stability: Refrigerated

Anti Thrombin III Activity   Stability: Room temp. 8 hrs., Frozen 1 month.

PT 20210 Gene Mutation   Stability: Refrigerated

Protein C Activity   Stability: Room temp 8 hrs., frozen (-20 C) 1 month.  

Protein S Free Antigen with Reflex   Stability: Frozen 14 days.

Performed Test Frequency

Monday, Wednesday and Friday

CPT(s)

81240 81241 83090 85300 85303 85305 85306 85390 85610 85613 85670 86146 X 3 86147 X 3 85597 IF INDICATED 85613 IF INDICATED 85730 IF INDICATED 85732 X2 IF INDICATED

Cerner Order Code

OB HCP

Acceptable Collection Tube(s)

Blue - Anti Thrombin III Activity

Gold SST or Red Top - Required - Beta 2 Glycoprotein and Cardiolipin Antibodies

Lavender - Factor V Leiden

GRN PST - Homocysteine

2 Blue - Lupus Anticoagulant

Blue - Protein C Activity

Blue - Protein S Free Antigen with Reflex

Lavender - PT 20210 Gene Mutation

Rejection Criteria

Homocysteine  Rejection criteria:  Gross hemolysis

Anti Thrombin III Activity   Rejection criteria:  refrigerated plasma.

Protein C Activity   Rejection criteria:  refrigerated plasma.

Protein S Free Antigen with Reflex   Rejection criteria:  Room temp, refrigerated, Gross hemolysis and lipemia.

Panel Includes

Beta 2 Glycoprotein and Cardiolipin Antibodies

Homocysteine

Lupus Anticoagulant

Factor V Leiden

Anti Thrombin III Activity

PT 20210 Gene Mutation

Protein C Activity

Protein S Free Antigen with Reflex

Performing Locations

Test performed at MercyOne Des Moines Laboratory and Mayo Clinic Laboratories, Rochester, MN.