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HelpTest Code CHRAF Chromosome Analysis, Amniotic Fluid
Additional Codes
Outreach (Atlas) Order Code
CHR AF
Hospital (Cerner) Order Code
CHRO AF
Useful For
Prenatal diagnosis of chromosome abnormalities, including aneuploidy (ie, trisomy or monosomy) and balanced rearrangements
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| _ML15 | Metaphases, <15 | No, (Bill Only) | No |
| _M15 | Metaphases, 15 | No, (Bill Only) | No |
| _MG14 | Metaphases, >15 | No, (Bill Only) | No |
| _COL1 | Colonies, 1-5 | No, (Bill Only) | No |
| _COL6 | Colonies, 6+ | No, (Bill Only) | No |
| _KTG1 | Karyotypes, >1 | No, (Bill Only) | No |
| _STAC | Ag-Nor/CBL Stain | No, (Bill Only) | No |
Testing Algorithm
This test is not appropriate as a first-tier test for detecting gains or losses of chromosomal material in pregnancies with 1 or more major structural abnormalities.
This test includes a charge for cell culture of fresh specimens and professional interpretation of results. Analysis charges will be incurred for total work performed, and generally include 2 banded karyograms and the analysis of 20 metaphase cells. If no metaphase cells are available for analysis, no analysis charges will be incurred. If additional analysis work is required, additional charges may be incurred.
Special Instructions
Method Name
Cell Culture followed by Chromosome Analysis
Reporting Name
Chromosomes, Amniotic FluidSpecimen Type
Amniotic FldOrdering Guidance
This test should be performed for prenatal diagnostic purposes only. A chromosomal microarray (CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling) is recommended, rather than chromosomal analysis, to detect clinically relevant gains or losses of chromosomal material in pregnancies with 1 or more major structural abnormalities. Chromosomal microarray can also be considered, rather than chromosome analysis, for patients undergoing invasive prenatal diagnostic testing with a structurally normal fetus.
Portions of the specimen may be used for other tests, such as measuring markers for neural tube defects (eg, AFPA / Alpha-Fetoprotein, Amniotic Fluid), molecular genetic testing, biochemical testing, and fluorescence in situ hybridization testing (including PADF / Prenatal Aneuploidy Detection, FISH). If additional molecular genetic or biochemical genetic testing is needed, order CULAF / Culture for Genetic Testing, Amniotic Fluid so amniocyte cultures may be set up specifically for the use in these tests.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
Provide a reason for referral and gestational age with each specimen and verify the specimen source. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
Specimen Required
Specimen Type: Amniotic fluid
Submission Container/Tube: Centrifuge tube
Specimen Volume: 20 to 25 mL
Collection Instructions:
1. Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted.
2. Discard the first 2 mL of amniotic fluid.
3. If ordering with PADF / Prenatal Aneuploidy Detection, FISH, submit a minimum of 14 mL.
4. If ordering with CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling, submit a minimum of 24 mL.
5. If ordering with both PADF and CMAP, then submit a minimum of 26 mL.
Additional Information:
1. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.
2. If the specimen does not grow in culture, the client will be notified within 7 days of receipt.
3. Bloody specimens are undesirable.
Specimen Type: Fetal body fluid
Container/Tube: Sterile tube
Specimen Volume: Entire specimen
Additional Information:
1. If the specimen does not grow in culture, the client will be notified within 7 days of receipt.
2. Clearly indicate on tube and paperwork that specimen is fetal body fluid.
Specimen Minimum Volume
The following are the minimum volumes when only this test is ordered:
Amniotic fluid: 12 mL
Fetal body fluid: See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Amniotic Fld | Refrigerated (preferred) | ||
| Ambient | |||
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
An interpretative report will be provided.
Day(s) Performed
Monday through Friday
Report Available
10 to 14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88235, 88291-Tissue culture for amniotic fluid or chorionic villus cells, Interpretation and report
88269 w/modifier 52-Chromosome analysis, in situ for amniotic fluid cells, <6 colonies, 1 karyotype with banding (if appropriate)
88269-Chromosome analysis, in situ for amniotic fluid cells, 6 or greater colonies, 1 karyotype with banding (if appropriate)
88267, 88285-Chromosome analysis, amniotic fluid or chorionic villus, greater than 15 cells, 1 karyotype with banding (if appropriate)
88267 w/modifier 52-Chromosome analysis, amniotic fluid or chorionic villus, <15 cells, 1 karyotype with banding (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CHRAF | Chromosomes, Amniotic Fluid | 62351-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 52297 | Result Summary | 50397-9 |
| 52299 | Interpretation | 69965-2 |
| 52298 | Result | 82939-0 |
| CG765 | Reason for Referral | 42349-1 |
| CG766 | Specimen | 31208-2 |
| 52300 | Source | 31208-2 |
| 52302 | Method | 85069-3 |
| 52301 | Banding Method | 62359-5 |
| 54640 | Additional Information | 48767-8 |
| 52303 | Released By | 18771-6 |
Forms
New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available: